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The standard cultivar, STV 825, likewise had a high Tmax of 46 Â ° C and the typical pollen germination was 53 % (Table 2). Cultivars that had greater Topt likewise had a higher pollen germination portion and kept a higher pollen germination even at high temperature levels.

Comparable pollen behaviour was observed in snake melon (Matlob and Kelly, 1973), corn (Binelli et al., 1985) and peanuts (Kakani et al., 2002). Recent studies with Brassica napus have actually suggested that reduced pollen germination instead of pollen viability under heat is the significant cause of low pollen fertility (Young et al., 2004). Prasad et al. Aloni et al.( 2001 )in bell pepper established a high connection in between in vitro pollen germination and fruit-set/seed-set under high-temperature conditions; this suggests that pollen germination might be a helpful tool for screening cultivar tolerance to heat. The capability of pollen to germinate and grow well at temperature levels above 30 Â ° C could be utilized as a tool to determine high-temperature tolerance in cotton cultivars. Additional research studies will be required to figure out the minimum number of germinated pollen grains needed to have reliable fertilization. In the existing research study, the membrane thermostability revealed as relative injury varied in between 35 and 73 %, however had a bad correlation with pollen criteria (Fig. 3). Recently, ur Rahman et al. 2004) also concluded that membrane thermostability is not a helpful parameter for discriminating high-temperature tolerance of cotton cultivars under ambient temperatures.

The genotypic differences for pollen germination and pollen tube growth recognized in this research study might be due to the variation in their pollen carbohydrate concentration. Studies have actually shown that carbohydrates are responsible for pollen development and, especially, pollen cytoplasmic carbs and sucrose are included in safeguarding pollen practicality during exposure and dispersal (Pacini et al., 1996) and for pollen germination, simple sugars are the main substrates (Stanley, 1971).